Thesis: "Natural Killer Cells Extraction from Used Leuko-Reduction Filters in Blood Transfusion"
Advisor: Prof. Ali Akbar Pourfathollah
University of Tehran, Tehran, Iran
Cell and Molecular Biology
Researches and Projects
Disturbances in NK Cells and Tumors' Protein-Protein Interaction Network, a Pan-Cancer Analysis
This is still an unpublished project.
Establishing Blood Transfusion waste products as a source of NK cells (Part of M.Sc. Thesis)
Our project was part of a series conducted by the Iranian Blood Transfusion Organization to introduce blood transfusion waste products as a sustainable and cost-effective source of bio-molecules and cells. This is particularly important in under-developed countries where fundings and resources are limited. Methods including flow cytometry, immune cells isolation, ELISA, cell culture, and killing assays were used. NK cells obtained by our approach is comparable to peripheral blood’s NK cells in term of viability, Sub-populational composition, and function.
rbioapi: User-Friendly R Interface to Biologic Web Services' API
The R package, rbioapi, is an interface to biological and medical databases and web services. This package provides consistency, a smooth learning curve for the users, and scalability for the developers. To date, rbioapi fully supports Enrichr, JASPAR, miEAA, PANTHER, Reactome, STRING, and UniProt. I aim to expand this list and make rbioapi as comprehensive as possible by inviting collaborations and contributions.
The R package, parapurrr, offers a simple yet fully customizable way to iteratively run functions in R using multiple CPU cores (instead of the default, one). The package parapurrr deliver that by bridging purrr to foreach package and its adaptors. All of purrr’s mapping functions and every foreach adaptor available in CRAN are supported.
Skills and Experiences
Flow cytometry, Mammalian cells culture, ELISA, qPCR, Immune cells isolation (FACS, MACS), Western blotting (familiar with), General genetics lab skills (RNA isolation, Transduction, Genes cloning, Gel electrophoresis, etc.), General biochemistry lab skills (Chromatography, Spectrophotometry, Density gradient centrifugation, etc.), Mouse handling
Bioinformatics and Data Science:
R programming language, RNA-seq analysis, Software development, High-throughput data analysis, Systems biology, Graphs and network analysis, Data processing, Machine learning, Big data, Bioconductor, Primer design
Git, Unix/Linux shell and Bash scripting, Cloud computing and working with remote servers, Python (familiar with), FlowJo, SPSS, SQL (familiar with), Microsoft Office
Moosa Rezwani, Ali Akbar Pourfathollah, Farshid Noorbakhsh, rbioapi: user-friendly R interface to biologic web services’ API, Bioinformatics, Volume 38, Issue 10, 15 May 2022, Pages 2952–2953, https://doi.org/10.1093/bioinformatics/btac172
Moosa Rezwani, Abdulbaset Mazarzaei, Zahra Abbasi-Malati, Ali Akbar Pourfathollah, Leukocyte-Reduction Filters as Reliable and Economic Source of Natural Killer Cells. Iranian Journal of Immunology, 2022 (Accepted, Manuscript ID IJI-2108-2158)