Thesis: "Natural Killer Cells Extraction from Used Leuko-Reduction Filters in Blood Transfusion"
Advisor: Prof. Ali Akbar Pourfathollah
University of Tehran, Tehran, Iran
Cell and Molecular Biology
Researches and Projects
Disturbances in NK Cells and Tumors' Protein-Protein Interaction Network, a Pan-Cancer Analysis
This is still an unpublished project.
Establishing Blood Transfusion waste products as a source of NK cells (Part of M.Sc. Thesis)
Our project was one of several projects carried out by the Iranian Blood Transfusion Organization with the primary goal of introducing blood transfusion waste products as a sustainable and economic source of bio-molecules and cells. This is particularly important in under-developed countries where fundings and resources are limited. Methods including flow cytometry, immune cells isolation, ELISA, cell culture, and killing assays were used. NK cells obtained by our method is comparable to peripheral blood's NK cells in term of viability, Sub-populational composition, and function.
rbioapi: User-Friendly R Interface to Biologic Web Services' API
We developed an R package, rbioapi, a consistent, user-friendly, and scalable interface to biological and medical databases and web services. To date, rbioapi fully supports Enrichr, JASPAR, miEAA, PANTHER, Reactome, STRING, and UniProt. We aim to expand this list and make rbioapi as comprehensive as possible. To this end, we developed an infrastructure that facilitates collaborations and the implementation of new services.
We developed a simple yet fully customizable way to run functions iteratively in R using multiple CPU cores (instead of the default, one). The package parapurrr achieves that by bridging purrr to foreach package and its adaptors. We added support for all purrr's map family functions and every foreach adaptor available in CRAN.
Skills and Experiences
Flow cytometry, Mammalian cells culture, ELISA, qPCR, Mouse handling, Immune cells isolation (FACS, MACS), Western blotting (familiar with), General genetics lab skills (RNA isolation, Transduction, Genes cloning, Gel electrophoresis, etc.), General biochemistry lab skills (Chromatography, Spectrophotometry, Density gradient centrifugation, etc.)
Bioinformatics and Data Science:
R programming language, RNA-seq analysis, Microarray analysis, Software development, High-throughput data analysis, Systems biology, Data processing, Machine learning, Graphs and network analysis, Bioconductor, Primer design
Git, Unix/Linux shell and Bash scripting, Cloud computing and working with remote servers, Python (familiar with), FlowJo, SPSS, SQL (familiar with), Microsoft Office
Moosa Rezwani, Ali Akbar Pourfathollah, Farshid Noorbakhsh, rbioapi: user-friendly R interface to biologic web services’ API, Bioinformatics, Volume 38, Issue 10, 15 May 2022, Pages 2952–2953, https://doi.org/10.1093/bioinformatics/btac172
Moosa Rezwani, Abdulbaset Mazarzaei, Zahra Abbasi-Malati, Ali Akbar Pourfathollah, Leukocyte-Reduction Filters as Reliable and Economic Source of Natural Killer Cells. Iranian Journal of Immunology, 2022 (Accepted, Manuscript ID IJI-2108-2158)